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A1603
Sigma
Alginate Lyase from Flavobacterium sp.
powder, ≥10,000 units/g solid
| Enzyme Commission (EC) Number: | 4.2.2.3 ( BRENDA | IUBMB ) |
| MDL number: | MFCD03454593 |
Description
| Biochem/physiol Actions | Breaks down alginate or alginic acid to smaller molecules and reduces viscosity. |
| Other Notes | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer |
| Preparation Note | Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH. |
| Unit Definition | One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C |
Properties
| form | powder |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| reference | Iwamoto, Y., et al., Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. Biosci. Biotechnol. Biochem. 65, 133, (2001) |
